① ʻO ka waena o ka fosfomycin, hiki ke hoʻohana ʻia e like me ka vulcanization accelerator, cosmetics (cream, lotion), mineral aila, paraffin emulsifier, biological buffer.
② Hoʻohana ʻia ma ke ʻano he mea hoʻoheheʻe kinoea, hoʻomākaukau i ka buffer, surfactant, emulsifier a me ka accelerator.Hoʻohana ʻia ia i nā synthes organik.
E hoʻopau iā Tris me kahi liʻiliʻi o ka wai mahu ʻelua (300-500ml), hoʻohui i ka HCl, hoʻoponopono i ka pH i 7.6 me HCl (1N) a i ʻole NaOH (1N), a hope e hoʻohui i ka wai mahu pālua i 1000ml.ʻO kēia wai ka wai mālama, mālama ʻia i loko o ka friji ma 4 ℃.
'Ōlelo Aʻo: ʻokoʻa ka waiwai PH o Tris-Hcl me ka mahana, no laila pono ke ana ʻia ma ka lumi wela, no laila ʻoi aku ka hilinaʻi o nā hopena i ana ʻia.
ʻAno hoʻopaʻa
He kumu nāwaliwali ʻo Tris me ka pKa o 8.1 ma ka lumi wela (25 ℃).Wahi a ke kumumanaʻo buffering, ʻo ka nui o ka buffering pono o Tris buffers aia ma waena o pH7.0 a me 9.2.
ʻO ka pH o ka hopena wai o Tris base ma kahi o 10.5, a hiki ke loaʻa ka buffer o ka waiwai pH ma o ka hoʻohui ʻana i ka waika hydrochloric e hoʻoponopono i ka waiwai pH i ka waiwai makemake.Eia naʻe, pono e uku ʻia ka manaʻo o ka wela ma ka pKa o Tris.Degere, ua emi ka waiwai PH e 0.03.
ʻO 1M Tris-HCl 6.8 a me 1.5M Tris-HCl 8.8 ka mea hoʻohana pinepine ʻia no SDS-PAGE.
ʻOiai ʻo TAE, TBE a me nā reagents ʻē aʻe i synthesized mai Tris ka mea hoʻohana pinepine ʻia no ka electrophoresis DNA, hoʻohana nui ʻia ʻo TE (pH8.0) no ka dissolution DNA.(ʻO TE ʻo Tris me EDTA.)
ʻAʻole hoʻohana nui ʻia nā buffers Tris ma ke ʻano he mea hoʻoheheʻe no nā waikawa nucleic a me nā protein, akā he nui nā hoʻohana nui.Hoʻohana ʻia ʻo Tris no ka ulu ʻana o ke aniani protein ma lalo o nā kūlana pH like ʻole.Hiki ke hoʻohana i ka ikaika ionic haʻahaʻa o Tris buffer no ka hoʻokumu ʻana i nā fibers waena o lamin i C. elegans.ʻO Tris kekahi o nā mea nui o ka protein electrophoresis buffers.Eia kekahi, he mea waena ʻo Tris i ka hoʻomākaukau ʻana i nā surfactants, vulcanization accelerators, a me kekahi mau lāʻau lapaʻau.Hoʻohana ʻia ʻo Tris ma ke ʻano he titration standard.
Ma keʻano o nā kiʻi a me nā kikokikona, hiki ke komo i kaʻike huahana, nā lawelawe a me nā pono.Hiki iā ia ke hele ma mua o ka huahana e hoʻohui i kekahi mau ʻike (nā manaʻo o ka mea kūʻai aku, hopohopo, a me nā mea ʻē aʻe), ka palekana o ke kaiapuni, nā laʻana manuahi, etc.
1. Ho'āʻoʻia nā laʻana me ka ukuʻole e hōʻoia i ka hoʻohana maʻamau o nā mea kūʻai.
2. E like me ka makemake o nā mea kūʻai like ʻole, liʻiliʻi i ka 100g, nui i nā tons o nā barela, hiki ke hoʻokō i nā koi o ka hoʻopili.
3. ʻO nā huaʻōlelo uku maʻalahi, hoʻoili kelepona a ʻae ʻia paha (e hoʻokō i nā koi hoʻokipa)
4. Hiki ke hāʻawiʻia ka lawe wikiwikiʻana, ka lā hoʻokahi a iʻole ka lā aʻe, ke kaʻina holoʻokoʻa o ka nānāʻana i kaʻike logistics, e hōʻoia i ka hoʻohana ponoʻana o nā mea kūʻai.
5. Hana maikaʻi ma hope o ke kūʻai aku, hoʻomanawanui a me ka akahele e hoʻoponopono i nā ʻano pilikia a pau e hiki mai ana, e like me nā pilikia maikaʻi, e hui pū me nā mea kūʻai aku e hana ikaika, mai pale i ke kuleana a me ka pane passive.
6. ʻO ka hui maikaʻi, ka maikaʻi o ka hana a me ka ʻike ʻoihana e hoʻomaha wale i nā mea kūʻai aku e pili ana i kā mākou huahana, akā manaʻo hoʻi he hilinaʻi kā mākou hui.
7. Kuleana kūʻokoʻa i waho e kūkulu i ka mākeke honua a me ka mana o ka brand brand honua.
8. ʻO ka ʻoiaʻiʻo, ka palapala i hoʻokumu ʻia, 20 mau makahiki o ka mōʻaukala ʻoihana a me ka inoa maikaʻi, e ʻoluʻolu nā mea kūʻai aku, pōmaikaʻi like a lanakila-lanakila, hui pū ʻana.